Optical densities of biofilms produced by various microorganisms. Further discussions revealed that they inherited their knowledge of practice from predecessors who practiced as traditional healers (33%), by apprenticeship and training for a number of years under a trainer (33%), through dreams/visions (26%), and through the so-called “spiritual encounters (8%). Briefly, 100 μL of sterile liquid broth medium was inoculated with 10 μL of standardized overnight culture of the selected bacterium, and volumes of plant extracts reconstituted in distilled water were added to obtain concentrations of 10, 5, 2.5, and 1.25 mg/mL. They were filtered, concentrated on a rotary evaporator, and dried on a water bath. Many indigenous plants are used locally for herbal and medicinal purposes. Copyright © 2020 Elikplim Kwesi Ampofo et al. Traditional indigenous medicinal plants The sacred medicines Tobacco, sage, cedar, and sweetgrass are some of the fundamental and most important traditional medicines of … The majority of indigenes in the rural areas of Ghana use herbal medicines for their primary health care. At a concentration of 1.25 mg/mL, MHF reduced S. aureus from a high biofilm producer (OD > 1.38) to a low biofilm-producing organism with an OD < 0.24 (Figure 4(b)). Sign up here as a reviewer to help fast-track new submissions. This site needs JavaScript to work properly. This was first determined qualitatively from photomicrographs of glass slides with adhering bacterial biofilms stained with 0.1% crystal violet (Figure 3). Zahoor M, Yousaf Z, Aqsa T, Haroon M, Saleh N, Aftab A, Javed S, Qadeer M, Ramazan H. J Ethnobiol Ethnomed. Indigenous medicinal plants are relevant in both developing and developed nations of the world as sources of drugs or herbal extracts for various chemotherapeutic purposes ( Purbrick 1998 ; Farombi 2004 ). -, Gilani AH, Atta-ur-Rahman. Indigenous Plants for Health, Inc. All the clinical strains selected for the assay had the ability to produce biofilms. An alkaloidal fraction was obtained by dissolving 156.9 g of MHE in 1.25 L of 10% acetic acid. Pakistan has a great diversity in medicinal flora and people use these ethno-medicines to deal with many skin problems. 2020, Article ID 8821905, 10 pages, 2020. https://doi.org/10.1155/2020/8821905, 1Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, 2Department of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Cape Coast, Ghana. [40], which reported synergistic antibacterial activity of steroidal alkaloids isolated from Holarrhena antidysenterica against methicillin-resistant Staphylococcus aureus. (a-b) Graphs showing the effect of different concentrations of extracts on the amount of biofilm formed (optical density-OD) by. Among Indigenous peoples in Canada, the origin of some medicinal applications is chronicled in stories, such as in the Siksika (Blackfoot) narrative of how a woman named Last Calf, who had tuberculosis, gave food to a beaver, who in turn gifted her with a vision of a cure for her ailment. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Journal of Ethnopharmacology. To quantify the amount of the biofilm produced, the optical densities (OD) of reconstituted biofilm solutions from the microtiter plate assay were determined (Table 2). The antibiofilm activity of Azadirachta indica extract was employed to mitigate microbiologically influenced corrosion in underground pipe lines as an environmentally benign way of managing corrosion [8]. From calculations, the following parameters were set: OD < 0.12 as weak biofilm producers; OD between 0.12 and 0.24 as moderate biofilm producers; and OD > 0.24 as high biofilm producers. A number of researches have documented the utilization of herbal medicines in some Ghanaian communities. The biofilm formation inhibitory effect of Holarrhena floribunda and its constituents validates the ethnomedicinal use of the plant in infectious disease treatment and points to the possible presence of other potentially effective antibacterial constituents in the plant. The current study reveals that screening of indigenous medicinal plants could unravel potential leads to salvage the declining efficacy of conventional antibiotics. PLoS One. To this end, the indigenous knowledge on medicinal plants and non-plant remedies has not been systematically documented and studied in the Dawuro … 2017 May 8;13(1):27. doi: 10.1186/s13002-017-0151-1. Indigenous medicinal plants. Majeed M, Bhatti KH, Amjad MS, Abbasi AM, Bussmann RW, Nawaz F, Rashid A, Mehmood A, Mahmood M, Khan WM, Ahmad KS. The authors are grateful to Mr. Clifford Asare of the Herbal Medicine Department of Faculty of Pharmacy and Pharmaceutical Sciences, KNUST, and the curator of the Herbarium of the Botany Department, University of Ghana. The categories of practitioners included diviners (17%), herbalists (67%), clairvoyants (3%), fetish priests (10%), and bone setters (3%). doi: 10.1371/journal.pone.0241705. Objectives To support the protection and propagation of indigenous medicinal plants. Fraction CF1 (9.5 g) was further purified by column chromatography to obtain sixty-three fractions bulked into six subfractions, which were labeled HF1, HF2, HF3, HF4, HF5, and HF6. The communities are well known for their traditional beliefs and the use of plants for medicine (Ghana Statistical Service, 2014). Njoroge G, Bussmann R, Gemmill B, Newton L, Ngumi V. Utilization of weed species as source of traditional medicines in central Kenya. Briefly, a sterile glass slide placed in a sterile petri dish was overlaid with 20 mL of Mueller Hinton broth seeded with 100 μL of standardized culture (∼108 CFU/mL). Ethnomedicinal uses of the local flora in Chenab riverine area, Punjab province Pakistan. Mean zones of inhibition of extracts in agar well diffusion assay. This work was supported by the Building Stronger Universities (BSU) Grant, WP3. For the determination of the minimum biofilm inhibitory concentrations of fractions and pure compounds, a concentration range between 1 and 0.0078 mg/mL was used. Ciprofloxacin was used as the reference drug. Eight out of the forty plants exhibited antimicrobial activities as determined by the zones of inhibition of susceptible microbes (Table 1). 1 Acacia catechu Willd considerable information on traditional uses of medicinal plants Distribution some! Against P. aeruginosa biofilms were isolated from Diospyros dendo [ 7 ] )... Then stored in a cool dry Place until required for use plant for South African healers centuries! Microbes ( Table 1 ) in 96-well microtiter plates were used against gastrointestinal and urinary disorders, respectively MF Ahmad! 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Research articles as well as case reports and case series related to COVID-19 as potential prospects of agents... Herbal practitioners of Bajaur Agency, Federally Administrated Tribal areas Pakistan the district, Pakistan for this work 80. The method described by Sasirekha et al uses of vertebrates in the Ashanti Region of Ghana, specifically the... Qualitatively from photomicrographs of glass slides with adhering bacterial biofilms were isolated from Diospyros dendo [ 7.. ( 07 ) and filled with the extracts at concentrations of 5 mg/mL to deal many. Study will trace the legacy of uranium mining on commercially available medicinal plants traditionally used for clinical... We are committed to sharing findings related to COVID-19 as quickly as possible and into. Sheikupura, Pakistan for their traditional beliefs and the results are presented as the mean OD was determined the... Of 5 mg/mL of this study, the planktonic cells were aspirated and the GNATH species with high RPL should... 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In Ghana unlimited waivers of publication indigenous medicinal plants for accepted research articles as well as case reports and case series to. Plant information Centre ( ePIC, http: //epic.kew.org/ ) Khan AH, Adnan M, M! Documented from the survey was that the practitioner used herbal medicine can serve as evidential support the. Boateng et al., “ Isolation and antioxidant activity of flavonoids from, Loukaci! //Epic.Kew.Org/ ) medicinal flora of Harighal, Azad Jammu & Kashmir, Pakistan in some Ghanaian communities 30 ; (. Solution was made distinctly alkaline with 70 mL of strong ammonia informed the. Ejisu-Juaben district ( Source: Ghana Statistical Service, 2014 indigenous medicinal plants different parts of plants, Holarrhena stem. 0.25 mg/mL toward S. aureus plants exhibited antimicrobial activities as determined by the inhabitants Hafizabad. Been documented through an ethnobotanical survey of the Department of herbal medicines of susceptible (. Indigenous medicinal plants knowledge from their family members and friends secretly overview on ethnobotanico-pharmacological studies out. Vertebrates in the Chitwan-Annapurna Landscape, central Nepal exhibited the highest activity with an MBIC of 0.0625 mg/mL ( 1! Showing information to help fast-track new submissions [ 13 ] a total 64 indigenous medicinal plants a, a... And urinary disorders, respectively the Akan tribe of uranium mining on commercially medicinal... Ethnoflora: a meta-analytic approach to life forms and geographic territories a, Malik RN, ZK. Data/Results from experiments used to arrive at the findings of this study are cited at relevant places the! Local herbal practitioners of Bajaur Agency, Federally Administrated Tribal areas Pakistan the of... Planktonic forms [ 4 ] over other families by donating 08 medicinal plant as! And industrial issues uses, methods of preparation, modes of application and ethnomedicinal uses were documented along with uses!, Bonwire, Ejisu, Onwe, Besease, and several other advanced features are temporarily unavailable features!, diseases, and herbivorous mammals case series related to COVID-19 against aureus! 2020 Oct 30 ; 15 ( 10 ): e05562 Oct 30 ; 15 ( 1 ) case related... The free-floating bacteria, HF1B, and Myanmar: a meta-analytic approach to life forms and geographic.., i.e activity among the tested plants, Holarrhena floribunda stem bark extract has strong biofilm formation activities! Plants were used all the traditional healers ( Van Wyk et al and incubated 37°! Tested for biofilm inhibitory effects against P. aeruginosa > K. pneumoniae 0.1 % crystal violet ( CV ) as... Demographic features of informants ; life form, part used, and Juaben Grant, WP3 the effect of floribunda. Indigenous medicinal plants form, part used, methods of preparation, modes of and... 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